b3 integrin-specific primers Search Results


integrin b3 mab  (Proteintech)
94
Proteintech integrin b3 mab
FIGURE 3. Upregulation of <t>integrin</t> aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and <t>b3</t> receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.
Integrin B3 Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+integrin-specific+primers/Integrin+beta-3+Antibody/pm30128492-57-121-124
Average 94 stars, based on 1 article reviews
integrin b3 mab - by Bioz Stars, 2026-09
94/100 stars
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antiangptl3 mab  (Proteintech)
91
Proteintech antiangptl3 mab
FIGURE 3. Upregulation of <t>integrin</t> aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and <t>b3</t> receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.
Antiangptl3 Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+integrin-specific+primers/ANGPTL3+Antibody/pm30128492-57-109-111
Average 91 stars, based on 1 article reviews
antiangptl3 mab - by Bioz Stars, 2026-09
91/100 stars
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anti bax mab  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti bax mab
FIGURE 3. Upregulation of <t>integrin</t> aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and <t>b3</t> receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.
Anti Bax Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+integrin-specific+primers/Bax+Rabbit+mAb/pm30128492-57-100-102
Average 96 stars, based on 1 article reviews
anti bax mab - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
08 22 2018 monoclonal antibody  (Proteintech)
96
Proteintech 08 22 2018 monoclonal antibody
FIGURE 3. Upregulation of <t>integrin</t> aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and <t>b3</t> receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.
08 22 2018 Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+integrin-specific+primers/IL-1+beta+Antibody/pm30128492-57-73-77
Average 96 stars, based on 1 article reviews
08 22 2018 monoclonal antibody - by Bioz Stars, 2026-09
96/100 stars
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anti p53 mab  (Proteintech)
96
Proteintech anti p53 mab
FIGURE 1. Inhibition of inflammation and apoptosis and VEGF levels both in vivo and in vitro among different groups. (A) Western blotting (left) and quantification (right) of IL-1b, IL-6, Bax, <t>P53,</t> and VEGF expression levels in the retinas of rats. (B) Real-time RT-PCR analysis of mRNA expression profiles in the retinas of rats. **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. (C) Western blotting (left) and RT-PCR quantification (right) of IL-1b, IL-6, Bax, P53, and VEGF expression levels in HRMECs. (D) Real-time RT-PCR analysis of mRNA expression profiles in HRMECs. **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF. (E) TUNEL assay for determining apoptosis rates in HRMECs in the following treatment groups: high glucose (HG), high glucose plus fenofibrate (HGþF), and normal glucose (NG). **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF.
Anti P53 Mab, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/b3+integrin-specific+primers/P53+Antibody/pm30128492-57-96-98
Average 96 stars, based on 1 article reviews
anti p53 mab - by Bioz Stars, 2026-09
96/100 stars
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Image Search Results


FIGURE 3. Upregulation of integrin aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and b3 receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.

Journal: Investigative ophthalmology & visual science

Article Title: Fenofibrate Exerts Protective Effects in Diabetic Retinopathy via Inhibition of the ANGPTL3 Pathway.

doi: 10.1167/iovs.18-24155

Figure Lengend Snippet: FIGURE 3. Upregulation of integrin aVb3 receptor expression in HRMECs after high-glucose stimulation (HG) and ANGPTL3 overexpression (OE) compared with normal glucose (NG) group and inhibition of inflammation and apoptosis and downregulation of VEGF levels in HRMECs after treatment with cilengitide, an integrin aVb3 receptor inhibitor (HGþC, OEþC). (A, B) Western blotting (top), quantification (bottom), and real-time RT-PCR analysis of expression profiles of integrin aV and b3 receptor levels in HRMECs. **P < 0.01 HG versus NG, ##P < 0.01 OE versus NG. Western blotting (C), quantification (D), analysis of protein, and real-time RT-PCR (E) analysis of mRNA expression profiles of inflammatory, apoptotic, and VEGF markers in HRMECs. **P < 0.01 HG versus HGþC. *P < 0.05 HG versus HGþC. ##P < 0.01 OE versus OEþC. #P < 0.01 OE versus OEþC.

Article Snippet: Primer Sequences Used for RT-PCR Analysis of Target Genes Gene Species Primer Sequence ANGPTL3 Human 50-CTCCTTCAGTTGGGACATGG-30/50-TCACTGGTTTGCAGCGATAG-30 Rats 50-ACACAGAGTCAGGGCTTTGG-30/50 -GACGTTACCTCTGGGTGCTC-30 IL-1b Human 50-TCCAGGGACAGGATATGGAG-30/50-TCTTTCAACACGCAGGACAG-30 Rats 50-TGACCCATGTGAGCTGAAAG-30/50-AGGGATTTTGTCGTTGCTTG-30 IL-6 Human 50-TCAGCCCTGAGAAAGGAGAC-30/50 -TTTCAGCCATCTTTGGAAGG-30 Rats 50-CCGGAGAGGAGACTTCACAG-30/50 -CAGAATTGCCATTGCACAAC-30 Bax Human 50-GGCCTCCTCTCCTACTTTGG-30/50 -CTCAGCCCATCTTCTTCCAG-30 Rats 50-GCTGGACACTGGACTTCCTC-30/50-CTCAGCCCATCTTCTTCCAG-30 P53 Human 50-GTTCCGAGAGCTGAATGAGG-30/50-TTATGGCGGGAGGTAGACTG-30 Rats 50-GCCATCTACAAGAAGTCACAGC-30/50 -CTCAGCCCATCTTCTTCCAG-30 VEGF Human 50-CCTTGCTGCTCTACCTCCAC-30/50 -AGCTGCGCTGATAGACATCC-30 Rats 50-GCTACTGCCGTCCGATTGAG-30/50-GCTGGCTTTGGTGAGGTTTG-30 Integrin aV Human 50-TCAGGACGGCACAAAGACC-30/50 -TACCAGGACC ACCGAGAAG-30 Rats 50-ACCAGGATGGCTTCAATGAC-30/50-AGCCTGTGGATCTTCCATTG-30 Integrin b3 Human 50-ATGGGGACACCTGTGAGAAG-30/50-AACGGTTGCAGGTATTTTCG-30 Rats 50-AAGGGCTGTCCTGTATGTGG-30/50-GAGTAGCAAGGCCAATGAGC-30 b-actin Human 50-GATCATTGCTCCTCCTGAGC-30/50 -ACATCTGCTGGAAGGTGGAC-30 Rats 50-TTGCTGACAGGATGCAGAAG-30/50-CAGTGAGGCCAGGATAGAGC-30 Downloaded From: https://iovs.arvojournals.org/ on 08/22/2018 monoclonal antibody (mAb; Proteintech, Rosemont, IL, USA, 16806-1-AP; Abcam, Cambridge, UK, ab9722), anti–IL-6 mAb (Bioworld Technology, Saint Louis Park, MN, USA, BS6419), anti-P53 mAb (Proteintech, 10442-1-AP), anti-Bax mAb (Cell Signaling Technology, Danvers, MA, USA, 14796), antiANGPTL3 mAb (Proteintech, 11964-1-AP; Abcam, ab175288), anti-integrin aV mAb (Abcam, ab150361), and integrin b3 mAb (Proteintech, 18309-1-AP). b-actin levels were detected using a mAb (Sigma-Aldrich, St. Louis, MO, USA, A2066) and used as an internal control to confirm equivalent total protein loading.

Techniques: Expressing, Over Expression, Inhibition, Western Blot, Quantitative RT-PCR

FIGURE 4. Downregulated expression of ANGPLT3, inflammation and apoptosis markers, and VEGF and integrin aVb3 receptors both in vivo and in vitro (A–C). Western blotting (A), quantification (B), and real-time RT-PCR analysis of the expression levels of (C) IL-1b, IL-6, Bax, P53, VEGF, ANGPTL3, and integrin aVb3 receptors in the retinas of rats in the following treatment groups: diabetic rats (DM), diabetic rats treated with fenofibrate (DMþF), intra-ocular injection with rhANGPTL3 rats with normal glucose (rhANG), intra-ocular injection with rhANGPTL3 rats with normal glucose plus fenofibrate (rhANGþF), rats exposed to normal glucose levels (NG). **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. ##P < 0.01 rhANG versus rhANGþF, #P < 0.05 rhANG versus rhANGþF. D-F. Western blotting (D), quantification (E), and real-time RT-PCR analysis

Journal: Investigative ophthalmology & visual science

Article Title: Fenofibrate Exerts Protective Effects in Diabetic Retinopathy via Inhibition of the ANGPTL3 Pathway.

doi: 10.1167/iovs.18-24155

Figure Lengend Snippet: FIGURE 4. Downregulated expression of ANGPLT3, inflammation and apoptosis markers, and VEGF and integrin aVb3 receptors both in vivo and in vitro (A–C). Western blotting (A), quantification (B), and real-time RT-PCR analysis of the expression levels of (C) IL-1b, IL-6, Bax, P53, VEGF, ANGPTL3, and integrin aVb3 receptors in the retinas of rats in the following treatment groups: diabetic rats (DM), diabetic rats treated with fenofibrate (DMþF), intra-ocular injection with rhANGPTL3 rats with normal glucose (rhANG), intra-ocular injection with rhANGPTL3 rats with normal glucose plus fenofibrate (rhANGþF), rats exposed to normal glucose levels (NG). **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. ##P < 0.01 rhANG versus rhANGþF, #P < 0.05 rhANG versus rhANGþF. D-F. Western blotting (D), quantification (E), and real-time RT-PCR analysis

Article Snippet: Primer Sequences Used for RT-PCR Analysis of Target Genes Gene Species Primer Sequence ANGPTL3 Human 50-CTCCTTCAGTTGGGACATGG-30/50-TCACTGGTTTGCAGCGATAG-30 Rats 50-ACACAGAGTCAGGGCTTTGG-30/50 -GACGTTACCTCTGGGTGCTC-30 IL-1b Human 50-TCCAGGGACAGGATATGGAG-30/50-TCTTTCAACACGCAGGACAG-30 Rats 50-TGACCCATGTGAGCTGAAAG-30/50-AGGGATTTTGTCGTTGCTTG-30 IL-6 Human 50-TCAGCCCTGAGAAAGGAGAC-30/50 -TTTCAGCCATCTTTGGAAGG-30 Rats 50-CCGGAGAGGAGACTTCACAG-30/50 -CAGAATTGCCATTGCACAAC-30 Bax Human 50-GGCCTCCTCTCCTACTTTGG-30/50 -CTCAGCCCATCTTCTTCCAG-30 Rats 50-GCTGGACACTGGACTTCCTC-30/50-CTCAGCCCATCTTCTTCCAG-30 P53 Human 50-GTTCCGAGAGCTGAATGAGG-30/50-TTATGGCGGGAGGTAGACTG-30 Rats 50-GCCATCTACAAGAAGTCACAGC-30/50 -CTCAGCCCATCTTCTTCCAG-30 VEGF Human 50-CCTTGCTGCTCTACCTCCAC-30/50 -AGCTGCGCTGATAGACATCC-30 Rats 50-GCTACTGCCGTCCGATTGAG-30/50-GCTGGCTTTGGTGAGGTTTG-30 Integrin aV Human 50-TCAGGACGGCACAAAGACC-30/50 -TACCAGGACC ACCGAGAAG-30 Rats 50-ACCAGGATGGCTTCAATGAC-30/50-AGCCTGTGGATCTTCCATTG-30 Integrin b3 Human 50-ATGGGGACACCTGTGAGAAG-30/50-AACGGTTGCAGGTATTTTCG-30 Rats 50-AAGGGCTGTCCTGTATGTGG-30/50-GAGTAGCAAGGCCAATGAGC-30 b-actin Human 50-GATCATTGCTCCTCCTGAGC-30/50 -ACATCTGCTGGAAGGTGGAC-30 Rats 50-TTGCTGACAGGATGCAGAAG-30/50-CAGTGAGGCCAGGATAGAGC-30 Downloaded From: https://iovs.arvojournals.org/ on 08/22/2018 monoclonal antibody (mAb; Proteintech, Rosemont, IL, USA, 16806-1-AP; Abcam, Cambridge, UK, ab9722), anti–IL-6 mAb (Bioworld Technology, Saint Louis Park, MN, USA, BS6419), anti-P53 mAb (Proteintech, 10442-1-AP), anti-Bax mAb (Cell Signaling Technology, Danvers, MA, USA, 14796), antiANGPTL3 mAb (Proteintech, 11964-1-AP; Abcam, ab175288), anti-integrin aV mAb (Abcam, ab150361), and integrin b3 mAb (Proteintech, 18309-1-AP). b-actin levels were detected using a mAb (Sigma-Aldrich, St. Louis, MO, USA, A2066) and used as an internal control to confirm equivalent total protein loading.

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Quantitative RT-PCR, Injection

FIGURE 1. Inhibition of inflammation and apoptosis and VEGF levels both in vivo and in vitro among different groups. (A) Western blotting (left) and quantification (right) of IL-1b, IL-6, Bax, P53, and VEGF expression levels in the retinas of rats. (B) Real-time RT-PCR analysis of mRNA expression profiles in the retinas of rats. **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. (C) Western blotting (left) and RT-PCR quantification (right) of IL-1b, IL-6, Bax, P53, and VEGF expression levels in HRMECs. (D) Real-time RT-PCR analysis of mRNA expression profiles in HRMECs. **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF. (E) TUNEL assay for determining apoptosis rates in HRMECs in the following treatment groups: high glucose (HG), high glucose plus fenofibrate (HGþF), and normal glucose (NG). **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF.

Journal: Investigative ophthalmology & visual science

Article Title: Fenofibrate Exerts Protective Effects in Diabetic Retinopathy via Inhibition of the ANGPTL3 Pathway.

doi: 10.1167/iovs.18-24155

Figure Lengend Snippet: FIGURE 1. Inhibition of inflammation and apoptosis and VEGF levels both in vivo and in vitro among different groups. (A) Western blotting (left) and quantification (right) of IL-1b, IL-6, Bax, P53, and VEGF expression levels in the retinas of rats. (B) Real-time RT-PCR analysis of mRNA expression profiles in the retinas of rats. **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. (C) Western blotting (left) and RT-PCR quantification (right) of IL-1b, IL-6, Bax, P53, and VEGF expression levels in HRMECs. (D) Real-time RT-PCR analysis of mRNA expression profiles in HRMECs. **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF. (E) TUNEL assay for determining apoptosis rates in HRMECs in the following treatment groups: high glucose (HG), high glucose plus fenofibrate (HGþF), and normal glucose (NG). **P < 0.01 HG versus HGþF, *P < 0.05 HG versus HGþF.

Article Snippet: Primer Sequences Used for RT-PCR Analysis of Target Genes Gene Species Primer Sequence ANGPTL3 Human 50-CTCCTTCAGTTGGGACATGG-30/50-TCACTGGTTTGCAGCGATAG-30 Rats 50-ACACAGAGTCAGGGCTTTGG-30/50 -GACGTTACCTCTGGGTGCTC-30 IL-1b Human 50-TCCAGGGACAGGATATGGAG-30/50-TCTTTCAACACGCAGGACAG-30 Rats 50-TGACCCATGTGAGCTGAAAG-30/50-AGGGATTTTGTCGTTGCTTG-30 IL-6 Human 50-TCAGCCCTGAGAAAGGAGAC-30/50 -TTTCAGCCATCTTTGGAAGG-30 Rats 50-CCGGAGAGGAGACTTCACAG-30/50 -CAGAATTGCCATTGCACAAC-30 Bax Human 50-GGCCTCCTCTCCTACTTTGG-30/50 -CTCAGCCCATCTTCTTCCAG-30 Rats 50-GCTGGACACTGGACTTCCTC-30/50-CTCAGCCCATCTTCTTCCAG-30 P53 Human 50-GTTCCGAGAGCTGAATGAGG-30/50-TTATGGCGGGAGGTAGACTG-30 Rats 50-GCCATCTACAAGAAGTCACAGC-30/50 -CTCAGCCCATCTTCTTCCAG-30 VEGF Human 50-CCTTGCTGCTCTACCTCCAC-30/50 -AGCTGCGCTGATAGACATCC-30 Rats 50-GCTACTGCCGTCCGATTGAG-30/50-GCTGGCTTTGGTGAGGTTTG-30 Integrin aV Human 50-TCAGGACGGCACAAAGACC-30/50 -TACCAGGACC ACCGAGAAG-30 Rats 50-ACCAGGATGGCTTCAATGAC-30/50-AGCCTGTGGATCTTCCATTG-30 Integrin b3 Human 50-ATGGGGACACCTGTGAGAAG-30/50-AACGGTTGCAGGTATTTTCG-30 Rats 50-AAGGGCTGTCCTGTATGTGG-30/50-GAGTAGCAAGGCCAATGAGC-30 b-actin Human 50-GATCATTGCTCCTCCTGAGC-30/50 -ACATCTGCTGGAAGGTGGAC-30 Rats 50-TTGCTGACAGGATGCAGAAG-30/50-CAGTGAGGCCAGGATAGAGC-30 Downloaded From: https://iovs.arvojournals.org/ on 08/22/2018 monoclonal antibody (mAb; Proteintech, Rosemont, IL, USA, 16806-1-AP; Abcam, Cambridge, UK, ab9722), anti–IL-6 mAb (Bioworld Technology, Saint Louis Park, MN, USA, BS6419), anti-P53 mAb (Proteintech, 10442-1-AP), anti-Bax mAb (Cell Signaling Technology, Danvers, MA, USA, 14796), antiANGPTL3 mAb (Proteintech, 11964-1-AP; Abcam, ab175288), anti-integrin aV mAb (Abcam, ab150361), and integrin b3 mAb (Proteintech, 18309-1-AP). b-actin levels were detected using a mAb (Sigma-Aldrich, St. Louis, MO, USA, A2066) and used as an internal control to confirm equivalent total protein loading.

Techniques: Inhibition, In Vivo, In Vitro, Western Blot, Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, TUNEL Assay

FIGURE 2. ANGPTL3-induced inflammation and apoptosis and VEGF upregulation in diabetes both in vivo and in vitro. (A) Western blotting (left) and quantification (right) of ANGPTL3, IL-1b, IL-6, Bax, P53, and VEGF protein expression levels in the retinas of rats in the following treatment groups: diabetic rats (DM), intraocular injection with rhANGPTL3 in euglycemic rats (rhANG), intra-ocular injection with siRNA plasmid targeting ANGPTL3 in diabetic rats (DMþsi), and normal rats (NG). (B) Real-time RT-PCR analysis of mRNA expression profiles in the retinas of rats. **P < 0.01 DM versus NG, *P < 0.05 DM versus NG, ##P < 0.01 rhANG versus NG, #P < 0.05 rhANG versus NG, &&P < 0.01 DM versus DMþsi, &P < 0.05 DM versus DMþsi. (C) Western blotting (left) and quantification (right) of ANGPTL3, IL-1b, IL-6, Bax, P53, and VEGF protein expression levels in HRMECs in the following treatment groups: high glucose stimulation (HG), overexpression of ANGPTL3 mRNA (OE), ANGPTL3 siRNA interference of HRMECs under high glucose stimulation (HGþsi), and normal glucose level (NG). (D) Real-time RT-PCR analysis of mRNA expression profiles in HRMECs. (E) TUNEL assay (left) and apoptotic rates (right) in HRMECs. **P < 0.01 HG versus NG, *P < 0.05 HG versus NG, ##P < 0.01 OE versus NG, #P < 0.05 OE versus NG, &&P < 0.01 HG versus HGþsi, &P < 0.05 HG versus HGþsi.

Journal: Investigative ophthalmology & visual science

Article Title: Fenofibrate Exerts Protective Effects in Diabetic Retinopathy via Inhibition of the ANGPTL3 Pathway.

doi: 10.1167/iovs.18-24155

Figure Lengend Snippet: FIGURE 2. ANGPTL3-induced inflammation and apoptosis and VEGF upregulation in diabetes both in vivo and in vitro. (A) Western blotting (left) and quantification (right) of ANGPTL3, IL-1b, IL-6, Bax, P53, and VEGF protein expression levels in the retinas of rats in the following treatment groups: diabetic rats (DM), intraocular injection with rhANGPTL3 in euglycemic rats (rhANG), intra-ocular injection with siRNA plasmid targeting ANGPTL3 in diabetic rats (DMþsi), and normal rats (NG). (B) Real-time RT-PCR analysis of mRNA expression profiles in the retinas of rats. **P < 0.01 DM versus NG, *P < 0.05 DM versus NG, ##P < 0.01 rhANG versus NG, #P < 0.05 rhANG versus NG, &&P < 0.01 DM versus DMþsi, &P < 0.05 DM versus DMþsi. (C) Western blotting (left) and quantification (right) of ANGPTL3, IL-1b, IL-6, Bax, P53, and VEGF protein expression levels in HRMECs in the following treatment groups: high glucose stimulation (HG), overexpression of ANGPTL3 mRNA (OE), ANGPTL3 siRNA interference of HRMECs under high glucose stimulation (HGþsi), and normal glucose level (NG). (D) Real-time RT-PCR analysis of mRNA expression profiles in HRMECs. (E) TUNEL assay (left) and apoptotic rates (right) in HRMECs. **P < 0.01 HG versus NG, *P < 0.05 HG versus NG, ##P < 0.01 OE versus NG, #P < 0.05 OE versus NG, &&P < 0.01 HG versus HGþsi, &P < 0.05 HG versus HGþsi.

Article Snippet: Primer Sequences Used for RT-PCR Analysis of Target Genes Gene Species Primer Sequence ANGPTL3 Human 50-CTCCTTCAGTTGGGACATGG-30/50-TCACTGGTTTGCAGCGATAG-30 Rats 50-ACACAGAGTCAGGGCTTTGG-30/50 -GACGTTACCTCTGGGTGCTC-30 IL-1b Human 50-TCCAGGGACAGGATATGGAG-30/50-TCTTTCAACACGCAGGACAG-30 Rats 50-TGACCCATGTGAGCTGAAAG-30/50-AGGGATTTTGTCGTTGCTTG-30 IL-6 Human 50-TCAGCCCTGAGAAAGGAGAC-30/50 -TTTCAGCCATCTTTGGAAGG-30 Rats 50-CCGGAGAGGAGACTTCACAG-30/50 -CAGAATTGCCATTGCACAAC-30 Bax Human 50-GGCCTCCTCTCCTACTTTGG-30/50 -CTCAGCCCATCTTCTTCCAG-30 Rats 50-GCTGGACACTGGACTTCCTC-30/50-CTCAGCCCATCTTCTTCCAG-30 P53 Human 50-GTTCCGAGAGCTGAATGAGG-30/50-TTATGGCGGGAGGTAGACTG-30 Rats 50-GCCATCTACAAGAAGTCACAGC-30/50 -CTCAGCCCATCTTCTTCCAG-30 VEGF Human 50-CCTTGCTGCTCTACCTCCAC-30/50 -AGCTGCGCTGATAGACATCC-30 Rats 50-GCTACTGCCGTCCGATTGAG-30/50-GCTGGCTTTGGTGAGGTTTG-30 Integrin aV Human 50-TCAGGACGGCACAAAGACC-30/50 -TACCAGGACC ACCGAGAAG-30 Rats 50-ACCAGGATGGCTTCAATGAC-30/50-AGCCTGTGGATCTTCCATTG-30 Integrin b3 Human 50-ATGGGGACACCTGTGAGAAG-30/50-AACGGTTGCAGGTATTTTCG-30 Rats 50-AAGGGCTGTCCTGTATGTGG-30/50-GAGTAGCAAGGCCAATGAGC-30 b-actin Human 50-GATCATTGCTCCTCCTGAGC-30/50 -ACATCTGCTGGAAGGTGGAC-30 Rats 50-TTGCTGACAGGATGCAGAAG-30/50-CAGTGAGGCCAGGATAGAGC-30 Downloaded From: https://iovs.arvojournals.org/ on 08/22/2018 monoclonal antibody (mAb; Proteintech, Rosemont, IL, USA, 16806-1-AP; Abcam, Cambridge, UK, ab9722), anti–IL-6 mAb (Bioworld Technology, Saint Louis Park, MN, USA, BS6419), anti-P53 mAb (Proteintech, 10442-1-AP), anti-Bax mAb (Cell Signaling Technology, Danvers, MA, USA, 14796), antiANGPTL3 mAb (Proteintech, 11964-1-AP; Abcam, ab175288), anti-integrin aV mAb (Abcam, ab150361), and integrin b3 mAb (Proteintech, 18309-1-AP). b-actin levels were detected using a mAb (Sigma-Aldrich, St. Louis, MO, USA, A2066) and used as an internal control to confirm equivalent total protein loading.

Techniques: In Vivo, In Vitro, Western Blot, Expressing, Injection, Plasmid Preparation, Quantitative RT-PCR, Over Expression, TUNEL Assay

FIGURE 4. Downregulated expression of ANGPLT3, inflammation and apoptosis markers, and VEGF and integrin aVb3 receptors both in vivo and in vitro (A–C). Western blotting (A), quantification (B), and real-time RT-PCR analysis of the expression levels of (C) IL-1b, IL-6, Bax, P53, VEGF, ANGPTL3, and integrin aVb3 receptors in the retinas of rats in the following treatment groups: diabetic rats (DM), diabetic rats treated with fenofibrate (DMþF), intra-ocular injection with rhANGPTL3 rats with normal glucose (rhANG), intra-ocular injection with rhANGPTL3 rats with normal glucose plus fenofibrate (rhANGþF), rats exposed to normal glucose levels (NG). **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. ##P < 0.01 rhANG versus rhANGþF, #P < 0.05 rhANG versus rhANGþF. D-F. Western blotting (D), quantification (E), and real-time RT-PCR analysis

Journal: Investigative ophthalmology & visual science

Article Title: Fenofibrate Exerts Protective Effects in Diabetic Retinopathy via Inhibition of the ANGPTL3 Pathway.

doi: 10.1167/iovs.18-24155

Figure Lengend Snippet: FIGURE 4. Downregulated expression of ANGPLT3, inflammation and apoptosis markers, and VEGF and integrin aVb3 receptors both in vivo and in vitro (A–C). Western blotting (A), quantification (B), and real-time RT-PCR analysis of the expression levels of (C) IL-1b, IL-6, Bax, P53, VEGF, ANGPTL3, and integrin aVb3 receptors in the retinas of rats in the following treatment groups: diabetic rats (DM), diabetic rats treated with fenofibrate (DMþF), intra-ocular injection with rhANGPTL3 rats with normal glucose (rhANG), intra-ocular injection with rhANGPTL3 rats with normal glucose plus fenofibrate (rhANGþF), rats exposed to normal glucose levels (NG). **P < 0.01 DM versus DMþF, *P < 0.05 DM versus DMþF. ##P < 0.01 rhANG versus rhANGþF, #P < 0.05 rhANG versus rhANGþF. D-F. Western blotting (D), quantification (E), and real-time RT-PCR analysis

Article Snippet: Primer Sequences Used for RT-PCR Analysis of Target Genes Gene Species Primer Sequence ANGPTL3 Human 50-CTCCTTCAGTTGGGACATGG-30/50-TCACTGGTTTGCAGCGATAG-30 Rats 50-ACACAGAGTCAGGGCTTTGG-30/50 -GACGTTACCTCTGGGTGCTC-30 IL-1b Human 50-TCCAGGGACAGGATATGGAG-30/50-TCTTTCAACACGCAGGACAG-30 Rats 50-TGACCCATGTGAGCTGAAAG-30/50-AGGGATTTTGTCGTTGCTTG-30 IL-6 Human 50-TCAGCCCTGAGAAAGGAGAC-30/50 -TTTCAGCCATCTTTGGAAGG-30 Rats 50-CCGGAGAGGAGACTTCACAG-30/50 -CAGAATTGCCATTGCACAAC-30 Bax Human 50-GGCCTCCTCTCCTACTTTGG-30/50 -CTCAGCCCATCTTCTTCCAG-30 Rats 50-GCTGGACACTGGACTTCCTC-30/50-CTCAGCCCATCTTCTTCCAG-30 P53 Human 50-GTTCCGAGAGCTGAATGAGG-30/50-TTATGGCGGGAGGTAGACTG-30 Rats 50-GCCATCTACAAGAAGTCACAGC-30/50 -CTCAGCCCATCTTCTTCCAG-30 VEGF Human 50-CCTTGCTGCTCTACCTCCAC-30/50 -AGCTGCGCTGATAGACATCC-30 Rats 50-GCTACTGCCGTCCGATTGAG-30/50-GCTGGCTTTGGTGAGGTTTG-30 Integrin aV Human 50-TCAGGACGGCACAAAGACC-30/50 -TACCAGGACC ACCGAGAAG-30 Rats 50-ACCAGGATGGCTTCAATGAC-30/50-AGCCTGTGGATCTTCCATTG-30 Integrin b3 Human 50-ATGGGGACACCTGTGAGAAG-30/50-AACGGTTGCAGGTATTTTCG-30 Rats 50-AAGGGCTGTCCTGTATGTGG-30/50-GAGTAGCAAGGCCAATGAGC-30 b-actin Human 50-GATCATTGCTCCTCCTGAGC-30/50 -ACATCTGCTGGAAGGTGGAC-30 Rats 50-TTGCTGACAGGATGCAGAAG-30/50-CAGTGAGGCCAGGATAGAGC-30 Downloaded From: https://iovs.arvojournals.org/ on 08/22/2018 monoclonal antibody (mAb; Proteintech, Rosemont, IL, USA, 16806-1-AP; Abcam, Cambridge, UK, ab9722), anti–IL-6 mAb (Bioworld Technology, Saint Louis Park, MN, USA, BS6419), anti-P53 mAb (Proteintech, 10442-1-AP), anti-Bax mAb (Cell Signaling Technology, Danvers, MA, USA, 14796), antiANGPTL3 mAb (Proteintech, 11964-1-AP; Abcam, ab175288), anti-integrin aV mAb (Abcam, ab150361), and integrin b3 mAb (Proteintech, 18309-1-AP). b-actin levels were detected using a mAb (Sigma-Aldrich, St. Louis, MO, USA, A2066) and used as an internal control to confirm equivalent total protein loading.

Techniques: Expressing, In Vivo, In Vitro, Western Blot, Quantitative RT-PCR, Injection